Investigation of the Structural Properties of Dopamine-Loaded Poly(butyl cyanoacrylate) Nanosystem

Jahansooz, Fatemeh; Ebrahimi Hosseinzade, Bahaman; Hatamian-Zarmi, Ashrafalsadat; Shahpasand, Koorosh

Investigation of the Structural Properties of Dopamine-Loaded Poly(butyl cyanoacrylate) Nanosystem

Document Type : Original Research

Authors

F. jahansooz ¹

B. ebrahimi hosseinzade ²

A. Hatamian-Zarmi ³

K. Shahpasand ³

¹ student

² assistant proffesor

³ assistant professor

Abstract

Research subject: Parkinson’s disease is a neurodegnerative disorder with no treatment due to the blood brain barrier (BBB) existence. The cure for this disease is Dopamine a chemical molecule.

Research approach: This study investigates biodegradable naoparticles (NPs) carrying dopamine (DA) across the blood–brain barrier. Ion polymerization and solvent methods were used to achieve this goal. Particle size, zeta potential, entrapment efficiency and in vitro drug release behavior, at pH 7 were examined.

Main results: The empty nanoparticles and drug-loaded nanoparticles were found to be spherical in shape and fluffy exterior, with mono-modal size distribution and negative zeta-potentials of increasing average sizes 90 to 120 nm simultaneously. Fourier transform infrared (FTIR) spectra demonstrated the polymerization of nBCA monomers and encapsulation of DA inside poly (butylcyanoacrylate) (PBCA).Thermal characteristics of the copolymer were investigated by Fourier-transform infrared spectroscopy (FTIR). Drug loading efficiency was around 25%.The in-vitro drug release profile of DA -loaded PBCA nanoparticles prepared from ion polymerization following solution techniques exhibited a gradual release; more than 20 ٪w/w of the drug was released after 51 h. The results showed that the DA–PBCA nanocapsules could be an effective carrier for hydrophilic agents. In this study, PBCA-NSPs were successfully generated as a delivery system for DA, providing a promising approach to improve the therapy of PDs.

Keywords

Poly (butylcyanoacrylate)

Dopamine

Parkinson’s disease

nano structure

polymerization

Subjects

Drug delivery

[1] Seppi, K., Ray Chaudhuri, K., Coelho, M., Fox, S.H., Katzenschlager, R., Perez Lloret, S., Weintraub, D., Sampaio, C., Collaborators of The Parkinson's Disease Update on Non‐Motor Symptoms Study Group on Behalf of The Movement Disorders Society Evidence‐Based Medicine Committee, Chahine, L. and Hametner, E.M., Update on Treatments For Nonmotor Symptoms of Parkinson's Disease—an Evidence‐Based Medicine Review. Movement Disorders, 34(2), pp.180-198. 2019.
[2] Süssmuth, S.D., Landfester, K., Tumani, H., Ludolph, A.C. and Brettschneider, J., Unsolved Medical Problems: Blood-Brain Barrier in Neurodegenerative Diseases: Perspectives for Nanomedicine. European Journal of Nanomedicine, 2(1), pp.39-47. 2009.
[3] Wu, M., Dellacherie, E., Durand, A.A. and Marie, E., Poly (n-Butyl Cyanoacrylate) Nanoparticles via Miniemulsion Polymerization (1): Dextran-based Surfactants. Colloids and Surfaces B: Biointerfaces, 69(1), pp.141-146. 2009.
[4] Obeso, J.A. and Olanow, W., Continuing Efforts to Obtain Continuous Delivery of Levodopa. Movement Disorders, 26(12), pp.2149-2150. 2011.
[5] You, Q., Hopf, T., Hintz, W., Rannabauer, S., Voigt, N., van Wachem, B., Henrich-Noack, P. and Sabel, B.A., Major Effects on Blood-retina Barrier Passage by Minor Alterations in Design of Polybutylcyanoacrylate Nanoparticles. Journal of Drug Targeting, 27(3), pp.338-346. 2019.
[6] Trapani, A., De Giglio, E., Cafagna, D., Denora, N., Agrimi, G., Cassano, T., Gaetani, S., Cuomo, V. and Trapani, G., Characterization and Evaluation of Chitosan Nanoparticles for Dopamine Brain Delivery. International Journal of Pharmaceutics, 419(1-2), pp.296-307. 2011.
[7] Rashed, E.R., Abd El‐Rehim, H.A. and El‐Ghazaly, M.A., Potential Efficacy of Dopamine Loaded‐PVP/PAA Nanogel in Experimental Models of Parkinsonism: Possible Disease Modifying Activity. Journal of Biomedical Materials Research Part A, 103(5), pp.1713-1720. 2015.
[8] Fan, C. and Wang, D.A., Novel Gelatin-Based Nano-Gels with Coordination-Induced Drug Loading for Intracellular Delivery. Journal of Materials Science & Technology, 32(9), pp.840-844. 2016.
[9] Qu, M., Lin, Q., He, S., Wang, L., Fu, Y., Zhang, Z. and Zhang, L., A Brain Targeting Functionalized Liposomes of the Dopamine Derivative N-3, 4-bis (pivaloyloxy)-Dopamine for Treatment of Parkinson's Disease. Journal of Controlled Release, 277, pp.173-182. 2018.
[10] Khan, M.S., Pandey, S., Talib, A., Bhaisare, M.L. and Wu, H.F., Controlled Delivery of Dopamine Hydrochloride Using Surface Modified Carbon Dots for Neuro Diseases. Colloids and Surfaces B: Biointerfaces, 134, pp.140-146. 2015.
[11] Masoudipour, E., Kashanian, S. and Maleki, N., A Targeted Drug Delivery System Based on Dopamine Functionalized Nano Graphene Oxide. Chemical Physics Letters, 668, pp.56-63. 2017.
[12] Han, M.G., Kim, S. and Liu, S.X., Synthesis and Degradation Behavior of Poly (Ethyl Cyanoacrylate). Polymer Degradation and Stability, 93(7), pp.1243-1251. 2008.
[13] Yordanov, G.G., Bedzhova, Z.A. and Dushkin, C.D., Preparation and Physicochemical Characterization of Novel Chlorambucil-Loaded anoparticles of poly (Butylcyanoacrylate). Colloid and Polymer Science, 288(8), pp.893-899. 2010.
[14] Hansali, F., Wu, M., Bendedouch, D. and Marie, E., N-Butyl Cyanoacrylate Miniemulsion Polymerization via the Phase Inversion Composition Method. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 393, pp.133-138. 2012.
[15] Voigt, N., Henrich-Noack, P., Kockentiedt, S., Hintz, W., Tomas, J. and Sabel, B.A., Surfactants, ot Size or Zeta-Potential Influence Blood–Brain Barrier Passage of Polymeric Nanoparticles. European Journal of pharmaceutics and Biopharmaceutics, 87(1), pp.19-29. 2014.
[16] Yordanov, G.G. and Dushkin, C.D., Preparation of Poly (Butylcyanoacrylate) Drug Carriers by Nanoprecipitation Using a Pre-synthesized Polymer and Different Colloidal Stabilizers. Colloid and Polymer Science, 288(9), pp.1019-1026. 2010.
[17] Yordanov, G., Evangelatov, A. and Skrobanska, R., Epirubicin Loaded to Pre-Polymerized Poly (Butyl cyanoacrylate) Nanoparticles: Preparation and In vitro Evaluation in Human Lung Adenocarcinoma Cells. Colloids and Surfaces B: Biointerfaces, 107, pp.115-123. 2013.
[18] Haghnazari, N., Mohammadi, H., Mokhtari, M.J., Karami, C. and Ghasemi, S., Synthesis and In vitro Cytotoxicity of a Novel Efficient Cisplatin-loaded Poly N-butyl cyanoacrylate. Indian Journal Of Pharmaceutical Education and Research, 50(1), pp.190-197. 2016.
[19] Ćurić, A., Möschwitzer, J.P. and Fricker, G., Development and Characterization of Novel Highly-Loaded Itraconazole Poly (Butyl cyanoacrylate) Polymeric Nanoparticles. European Journal of Pharmaceutics and Biopharmaceutics, 114, pp.175-185. 2017.
[20] Shahmabadi, H.E., Movahedi, F., Esfahani, M.K.M., Alavi, S.E., Eslamifar, A., Anaraki, G.M. and Akbarzadeh, A., Efficacy of Cisplatin-Loaded Polybutyl Cyanoacrylate Nanoparticles on the Glioblastoma. Tumor Biology, 35(5), pp.4799-4806. 2014.
[22] López, T., Bata-Garcia, J.L., Esquivel, D., Ortiz-Islas, E., Gonzalez, R., Ascencio, J., Quintana, P., Oskam, G., Alvarez-Cervera, F.J., Heredia-Lopez, F.J. and Gongora-Alfaro, J.L., Treatment of Parkinson’s Disease: Nanostructured Sol–gel Silica–Dopamine Reservoirs for Controlled Drug Release in the Central Nervous System. International Journal of Nanomedicine, 6, p.19. 2011.
[23] Bagad, M. and Khan, Z.A., Poly (N-butylcyanoacrylate) Nanoparticles for Oral Delivery of Quercetin: Preparation, Characterization, and Pharmacokinetics and Biodistribution Studies in Wistar Rats. International Journal of Nanomedicine, 10, p.3921. 2015.