Volume 7, Issue 3 (2023)
IQBQ 2023, 7(3): 49-66 |
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Naderi A, Mousavi S M. Evaluating the Metabolic Activity of Aspergillus niger for Organic Acid Production Considering the Impact of Small Molecules in Culture Media. IQBQ 2023; 7 (3) :49-66
URL: http://arcpe.modares.ac.ir/article-38-74842-en.html
URL: http://arcpe.modares.ac.ir/article-38-74842-en.html
1- Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran
2- Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran ,mousavi_m@modares.ac.ir
2- Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, Tehran, Iran ,
Abstract: (82 Views)
Research subject: Aspergillus niger stands as a versatile filamentous fungus renowned for its industrial significance in producing various organic acids, notably citric acid and oxalic acid. Low sugar concentration as substrate leads to the production of oxalic acid, therefore, this article delves into the intricate metabolic machinery orchestrating the synthesis of these acids within A. niger, shedding light on the pivotal role of culture media composition and metabolic activity.
Research approach: Through a comprehensive review of A. niger metabolism, this study elucidates the pathways involved in the biosynthesis of citric acid and oxalic acid, unraveling the intricate interplay of enzymatic cascades and regulatory mechanisms governing their production. Furthermore, it explores the impact of small molecules on metabolic flux through regulatory media, offering insights into strategies for controlling metabolic flux in order to eliminate oxalic acid production and amplify the citric acid production considering low sugar content of 30 g/l.
Main results: After careful review of previous researches, key reactions and genes was found and introduced for future researches in order to control the A. niger products. Examination of small molecule as a regulator in culture media not only elucidated the importance of culture media composition but also employing them helped us to redirect flux from oxalate toward citrate. NH4, Leucine, Cysteine, NaF, Glutathione, and Metformin were all found to be effective in the elimination of oxalic acid. In this regard, employing them leads to the production of 1868, 1530, 2093, 2250, 787, and 675 mg/L oxalic acid in comparison to the control culture media in which 5560 mg/L oxalic was produced. In addition, elimination of oxalic acid in some cases leads to the production of more acids like the culture containing NH4, Cysteine and Metformin.
Research approach: Through a comprehensive review of A. niger metabolism, this study elucidates the pathways involved in the biosynthesis of citric acid and oxalic acid, unraveling the intricate interplay of enzymatic cascades and regulatory mechanisms governing their production. Furthermore, it explores the impact of small molecules on metabolic flux through regulatory media, offering insights into strategies for controlling metabolic flux in order to eliminate oxalic acid production and amplify the citric acid production considering low sugar content of 30 g/l.
Main results: After careful review of previous researches, key reactions and genes was found and introduced for future researches in order to control the A. niger products. Examination of small molecule as a regulator in culture media not only elucidated the importance of culture media composition but also employing them helped us to redirect flux from oxalate toward citrate. NH4, Leucine, Cysteine, NaF, Glutathione, and Metformin were all found to be effective in the elimination of oxalic acid. In this regard, employing them leads to the production of 1868, 1530, 2093, 2250, 787, and 675 mg/L oxalic acid in comparison to the control culture media in which 5560 mg/L oxalic was produced. In addition, elimination of oxalic acid in some cases leads to the production of more acids like the culture containing NH4, Cysteine and Metformin.
Keywords: Aspergillus niger, Regulatory defined medium, Metabolic activity, Organic acid, Small molecule
Article Type: Original Research |
Subject:
Metabolic Engineering
Received: 2024/04/27 | Accepted: 2024/06/3 | Published: 2023/11/1
Received: 2024/04/27 | Accepted: 2024/06/3 | Published: 2023/11/1
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