The control of bacterial diseases of fish has always been one of the most important problems of aquaculteriets in recent years. One of these problems is bacterial resistance. Using antibiotics can cause problems for consumers. Therefore, the use of new antimicrobial agents with minimal complications is common. In the present study, using the acid-thermal method, the bentonite structure was modified and the chitosan and bentonite hybrids were modified in the stage of development. The structure of the compounds was studied using the Infrared Fourier Transform Spectrometry (FTIR), Surface Electron Scanning Microscopy (FE-SEM) and X-ray diffraction spectroscopy (EDX). Antimicrobial activity of two types of modified bentonite on gram negative bacterium Aeromonas hydrophila was studied in vitro using two methods of disk diffusion and microdilution. The results of the disc diffusion method showed that the antimicrobial compounds produced had antibacterial properties in experimental conditions against the Aeromonas hydrophilia bacteria. Antimicrobial activity (MIC and MBC) was determined using microdilution and clonal counting in Agar culture medium. The results showed that the modified compound with the acidic method had MBC of 28.57 and 14.28 mg / L for Aeromonas hydrophila bacteria in 10 minutes and 30 minutes. For chitosan and modified bentonite composite, MBC was 14.28 mg / ml for Aeromonas hydrophila bacteria in 10 and 30 minutes. The results of this study showed that modified bentonite and composite compounds have strong antimicrobial effects and can remove pathogens in laboratory conditions and can be used as new compounds to control pathogens in breeding systems.

 

Keywords: Chitosan Composite, Bentonite, Acid-Thermal Method, Aeromonas hydrophila, Antimicrobial Effect

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